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dna spotting solution  (CapitalBio Corporation)

 
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    CapitalBio Corporation dna spotting solution
    ( A ) Schematic of the i-chip (left). The i-chip consisted of a 3D-printed sample preparation unit and a microfluidic amplification unit. The sample preparation unit was designed to perform the entire process of POC <t>DNA</t> pretreatment using a basket module, a rotary valve, and a block module. The basket module had a DNA extraction disc at its bottom (dotted box). ( B ) Schematic of the microfluidic amplification unit. The microfluidic amplification unit consisted of a microfluidic channel layer and <t>an</t> <t>aldehyde-functionalized</t> glass chip. The probe channel layer (marked in red) was used to modify the capture probes on the aldehyde-functionalized chip. The microfluidic channel layer (marked in pink) was then assembled onto the chip to complete the amplification unit. The cross section of the probe fluidic and microfluidic channels constituted the reaction zone (dotted box). The numbers 1, 2, 3, and 4 on the probe channel layer indicated different probe channels with different colors. ( C ) Detection procedure of POCKET system. DNA was absorbed and extracted using the extraction disc at the bottom of the basket. Target DNA was purified using a wash buffer and amplified by RPA to obtain biotin-modified amplicons. The amplicons were bound to SA-AuNPs and captured by premodified capture probes to achieve a secondary signal amplification. The tertiary amplification was initiated by depositing silver ions onto the AuNPs. ( D ) The POCKET platform was scalable for parallel sample detections by i-chips assembly. ( E ) Schematic of the f-box, which was designed to display the results. This included a foldable cradle, a smartphone, an optical unit with a macro-lens, and a LED lamp. Photo credit (D): Huan Xu, Army Medical University.
    Dna Spotting Solution, supplied by CapitalBio Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna spotting solution/product/CapitalBio Corporation
    Average 90 stars, based on 1 article reviews
    dna spotting solution - by Bioz Stars, 2026-04
    90/100 stars

    Images

    1) Product Images from "An ultraportable and versatile point-of-care DNA testing platform"

    Article Title: An ultraportable and versatile point-of-care DNA testing platform

    Journal: Science Advances

    doi: 10.1126/sciadv.aaz7445

    ( A ) Schematic of the i-chip (left). The i-chip consisted of a 3D-printed sample preparation unit and a microfluidic amplification unit. The sample preparation unit was designed to perform the entire process of POC DNA pretreatment using a basket module, a rotary valve, and a block module. The basket module had a DNA extraction disc at its bottom (dotted box). ( B ) Schematic of the microfluidic amplification unit. The microfluidic amplification unit consisted of a microfluidic channel layer and an aldehyde-functionalized glass chip. The probe channel layer (marked in red) was used to modify the capture probes on the aldehyde-functionalized chip. The microfluidic channel layer (marked in pink) was then assembled onto the chip to complete the amplification unit. The cross section of the probe fluidic and microfluidic channels constituted the reaction zone (dotted box). The numbers 1, 2, 3, and 4 on the probe channel layer indicated different probe channels with different colors. ( C ) Detection procedure of POCKET system. DNA was absorbed and extracted using the extraction disc at the bottom of the basket. Target DNA was purified using a wash buffer and amplified by RPA to obtain biotin-modified amplicons. The amplicons were bound to SA-AuNPs and captured by premodified capture probes to achieve a secondary signal amplification. The tertiary amplification was initiated by depositing silver ions onto the AuNPs. ( D ) The POCKET platform was scalable for parallel sample detections by i-chips assembly. ( E ) Schematic of the f-box, which was designed to display the results. This included a foldable cradle, a smartphone, an optical unit with a macro-lens, and a LED lamp. Photo credit (D): Huan Xu, Army Medical University.
    Figure Legend Snippet: ( A ) Schematic of the i-chip (left). The i-chip consisted of a 3D-printed sample preparation unit and a microfluidic amplification unit. The sample preparation unit was designed to perform the entire process of POC DNA pretreatment using a basket module, a rotary valve, and a block module. The basket module had a DNA extraction disc at its bottom (dotted box). ( B ) Schematic of the microfluidic amplification unit. The microfluidic amplification unit consisted of a microfluidic channel layer and an aldehyde-functionalized glass chip. The probe channel layer (marked in red) was used to modify the capture probes on the aldehyde-functionalized chip. The microfluidic channel layer (marked in pink) was then assembled onto the chip to complete the amplification unit. The cross section of the probe fluidic and microfluidic channels constituted the reaction zone (dotted box). The numbers 1, 2, 3, and 4 on the probe channel layer indicated different probe channels with different colors. ( C ) Detection procedure of POCKET system. DNA was absorbed and extracted using the extraction disc at the bottom of the basket. Target DNA was purified using a wash buffer and amplified by RPA to obtain biotin-modified amplicons. The amplicons were bound to SA-AuNPs and captured by premodified capture probes to achieve a secondary signal amplification. The tertiary amplification was initiated by depositing silver ions onto the AuNPs. ( D ) The POCKET platform was scalable for parallel sample detections by i-chips assembly. ( E ) Schematic of the f-box, which was designed to display the results. This included a foldable cradle, a smartphone, an optical unit with a macro-lens, and a LED lamp. Photo credit (D): Huan Xu, Army Medical University.

    Techniques Used: Sample Prep, Amplification, Blocking Assay, DNA Extraction, Purification, Modification



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    Image Search Results


    ( A ) Schematic of the i-chip (left). The i-chip consisted of a 3D-printed sample preparation unit and a microfluidic amplification unit. The sample preparation unit was designed to perform the entire process of POC DNA pretreatment using a basket module, a rotary valve, and a block module. The basket module had a DNA extraction disc at its bottom (dotted box). ( B ) Schematic of the microfluidic amplification unit. The microfluidic amplification unit consisted of a microfluidic channel layer and an aldehyde-functionalized glass chip. The probe channel layer (marked in red) was used to modify the capture probes on the aldehyde-functionalized chip. The microfluidic channel layer (marked in pink) was then assembled onto the chip to complete the amplification unit. The cross section of the probe fluidic and microfluidic channels constituted the reaction zone (dotted box). The numbers 1, 2, 3, and 4 on the probe channel layer indicated different probe channels with different colors. ( C ) Detection procedure of POCKET system. DNA was absorbed and extracted using the extraction disc at the bottom of the basket. Target DNA was purified using a wash buffer and amplified by RPA to obtain biotin-modified amplicons. The amplicons were bound to SA-AuNPs and captured by premodified capture probes to achieve a secondary signal amplification. The tertiary amplification was initiated by depositing silver ions onto the AuNPs. ( D ) The POCKET platform was scalable for parallel sample detections by i-chips assembly. ( E ) Schematic of the f-box, which was designed to display the results. This included a foldable cradle, a smartphone, an optical unit with a macro-lens, and a LED lamp. Photo credit (D): Huan Xu, Army Medical University.

    Journal: Science Advances

    Article Title: An ultraportable and versatile point-of-care DNA testing platform

    doi: 10.1126/sciadv.aaz7445

    Figure Lengend Snippet: ( A ) Schematic of the i-chip (left). The i-chip consisted of a 3D-printed sample preparation unit and a microfluidic amplification unit. The sample preparation unit was designed to perform the entire process of POC DNA pretreatment using a basket module, a rotary valve, and a block module. The basket module had a DNA extraction disc at its bottom (dotted box). ( B ) Schematic of the microfluidic amplification unit. The microfluidic amplification unit consisted of a microfluidic channel layer and an aldehyde-functionalized glass chip. The probe channel layer (marked in red) was used to modify the capture probes on the aldehyde-functionalized chip. The microfluidic channel layer (marked in pink) was then assembled onto the chip to complete the amplification unit. The cross section of the probe fluidic and microfluidic channels constituted the reaction zone (dotted box). The numbers 1, 2, 3, and 4 on the probe channel layer indicated different probe channels with different colors. ( C ) Detection procedure of POCKET system. DNA was absorbed and extracted using the extraction disc at the bottom of the basket. Target DNA was purified using a wash buffer and amplified by RPA to obtain biotin-modified amplicons. The amplicons were bound to SA-AuNPs and captured by premodified capture probes to achieve a secondary signal amplification. The tertiary amplification was initiated by depositing silver ions onto the AuNPs. ( D ) The POCKET platform was scalable for parallel sample detections by i-chips assembly. ( E ) Schematic of the f-box, which was designed to display the results. This included a foldable cradle, a smartphone, an optical unit with a macro-lens, and a LED lamp. Photo credit (D): Huan Xu, Army Medical University.

    Article Snippet: Then, 5 μM amino-functionalized DNA probes (Tsingke, China) in DNA spotting solution (CapitalBio, China) were then injected into each channel and incubated for 2 hours at room temperature to modify the probes on the surface of glass chip through aldimine condensation.

    Techniques: Sample Prep, Amplification, Blocking Assay, DNA Extraction, Purification, Modification